Process for producing lipase



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y uN-I n sTATas fPArENT OFFIC -Q 'snozo nmomo, or osaxA-ru, rerun.

' rnocnss roe raonucme mrasn.

no Drawing. Application filed June a, 1925, Serial N0.'86,035,'and ma -is ue 1a. 1924.

My invention relates tea-process for pro:

ducinglipase for use in decomposing oil and fat, and has'for its object to obtain a'more or less pure lipase fromvegetable protein containingmaterial such as the refuse from beans after the extraction of the oil there from or oil cake or the like, with high efliciency of production andat a 1ow cost.-

It has heretofore been impossible to obtain 10 or prepare a pure lipase although-lipase bearing material has been'prepa'red from the residue separated by filtration :from: the

- product prepared byaddin dilute acetic acid to castor bean refuse an then grinding the compound. 'I 1 Diseases of he human stomach or -intes tines are seldom caused .by eatin rice, wheat of the like A farlarger'num er of these diseases are due to indigestion or. apep rego sulting from' oil or vfatcontaining f as for example fish, meat, fowl or fried foods.-

Such indigestion can be avoided by the use of I lipasewhich decom ses'oil and fat. .Moreover, both oil-and; at have a superior nntri tive or calorific value compared toall other kinds ofjoods, and theperfect digestion of oil or fat "can be attained bythe: aid .of

j lipase.

' The 'A, can be absorbed .by the stomach or inrtestines only vwhen the oil or fat containing itis completely decomposed. The aforesai flresidue containing lipase has been 'used for decomposingthese oils and fats, but it is obvious that a completely eflective action of lipase can only be obtained by use'of the free or pure lipase such as is obtainedg-hy means of my invention; v 1 I According to my: invention, refuse such as 40 vegetable'seeds; rich oil or fat, is i fe r-" mented at "a temperature 1 which causes propagation of perisporia'wlrich is rich in f lipase, The 1 ferment -is then dissolved comprises subjecting lipase teinaceous material richino11 and fat-1:01 fermentation at about 30 C. to 40 C. until water, the precipitate removed from e5 the-solutionand finallyacetic acid is added to the saidsolution and the lipaseprecipitated.

For the purpose of a more complete under standing of my invention I will explain be low a preferred practical process embodying my invention.

Castor bean refuse and soya bean refuse recently discovered nutrient vitamin are mixed in vessel in a'proportion of 1 to 20. The two kinds of refuse are mix in order toobtain a higher speed of fermentation, and a better appearance of the product. The ratioof. the mixing may be.

v The materials are'thoroughly mixed and then subjected to and maintained at a 'constant temperature,say 30 C. to 40C., for

a period of one' week or so. At the" end of the period the proteins in the soya bean refuse become a yeast-like substance by fermentation, which substance is immersedmwater, agitated therein andthen left for one or two hours. Afterthis. treatment the yeast-like substance dissolves -and non-fermented substances precipitate; The solution thus obtained is then introducedinto another "j vessel, and the lipase may .beprecipitatedby adding a quantity of acetic a'cidor-byevaporating the same at a low temperature.

Alternatively. the lipase can be obtained by subjecting one part of casto'r bean-refuse to a fermentation for five da s, then addin twenty parts of soya bean re thereto an subjecting the mixtureto fermentation for,

two or three days.

This process diminishes the volume of the.

chamber used for the fermentation or the fermentation chamber is utilized at a higher 'efiiciency than in the former-practice.

My experiments have'sho'wn that a prod- ,uct containing sevenhundredtimes the amount of lipaseoriginally contained in thethis process material used can be obtained Having" now particularly; escribed and ascertained the 'natureof my saidinvfention andin what manner the same isto be per-- formed, I declare that whatI claim is:-

1. The process of producing lipase which yielding pm- .the protein materialis converted into a yeastlike substance, immersing the .materi in water to. dissolve the yeast-like substance, liemoving l dissolved material, and 1;} v after treatlng the solutionth to precipitate hpas.

2. The'proc'ess as in claim 1 in which the protelnaceous material 1s castor bean refuse.

3. The processof producing lipase whichcomprises subjecting castor bean refuse to 5 fermentation, adding soya hean refuse thereto and continuing the fermentation at about 30 C. to 40 C. until protein material in the refuse is converted into a yeast-like substance, immersing the material irl water to dissolve the yeast-like substance, removing 10 the undissolved .material,' and thereafter treating the solution with acetic acid to precipitate lipase. r In testimony whereof I afiix my signature.

' SHOZO YAMAMOTO. 

